General information: The SALSA MLPA
Probemix P088 Oligodendroglioma 1p-19q is a
research use only (RUO) assay for the detection of deletions and duplications in the 1p and 19q chromosomal regions which are associated with oligodendroglioma. This probemix can also be used to detect the presence of p.R132C and p.R132H point mutations in
IDH1 gene and p.R172K and p.R172M point mutations in
IDH2 gene.
Oligodendrogliomas are central nervous system neoplasms derived from a subset of glial cells, known as oligodendrocytes. The diagnosis of oligodendroglioma is based on the presence of both an
IDH gene family mutation in combination with co-deletion of 1p and 19q arms (Louis et al. 2016).
Probemix content: The SALSA MLPA Probemix P088-C2 Oligodendroglioma 1p-19q contains 58 MLPA probes with amplification products between 122 and 504 nucleotides (nt). This includes 19 probes for the 1p arm plus three flanking probes for 1q arm, and 11 probes for the 19q arm plus two flanking probes for 19p arm. Furthermore, it also contains four probes specific for the p.R132H and p.R132C mutation of
IDH1 and for the p.R172K and p.R172M mutation of
IDH2 which will only generate a signal when the mutation is present. In addition, 14 reference probes are included and target relatively copy number stable regions in central nervous system tumours, especially in oligodendrogliomas. Complete probe sequences and the identity of the genes detected by the reference probes is available in Table 2b of the Product Description.
This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA quantity fragments (Q-fragments), two DNA denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at
www.mlpa.com.
SALSA Binning DNA SD054: The SD054 Binning DNA provided with this probemix can be used as Binning DNA sample for binning of four mutation-specific probes (203 nt probe 19529-L16492
IDH1 p.R132H=c.395G>A mutation, 227 nt probe 14787-L23353
IDH1 p.R132C=c.394C>T mutation, 238 nt probe 20963-L29002
IDH2 p.R172K=c.515G>A mutation and 244 nt probe 20963-L29001
IDH2 p.R172M=c.515G>T mutation). SD054 Binning DNA is a mixture of genomic DNA from healthy individuals and synthetic DNA that contains the target sequence detected by the above mentioned probes. Inclusion of one reaction with 5 μl SD054 Binning DNA in initial MLPA experiments is essential as it can be used to aid in data binning of the peak pattern using Coffalyser.Net software. Furthermore, Binning DNA should be included in the experiment whenever changes have been applied to the set-up of the capillary electrophoresis device (e.g. when capillaries have been renewed). Binning DNA should never be used as a reference sample in the MLPA data analysis, neither should it be used in quantification of mutation signals, as for this purpose true mutation positive patient samples or cell lines should be used. It is strongly advised to use DNA sample and reference DNA samples extracted with the same method and derived from the same source of tissue. For further details, please consult the SD054 Binning DNA product description.
This product is for research use only (RUO).
Sample DNA
Sample DNA developed for this product: